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Product Details:
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Sensitivity: | 0.2ppb | Incubation Temperature: | 25℃ |
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Incubation Time: | 30min—15min | Sample Performance: | Tissue |
Shelf Life: | 12 Months When Properly Stored | Specifications: | 96 Wells/kit |
MOQ: | 1 Kit | ||
High Light: | Chlortetracyline ELISA kit,antibiotic test kit,food safet elisa kit |
Chlortetracyline ELISA Test Kit
Catalog No. LSY-10049
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Chlortetracyline in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Chlortetracyline in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Chlortetracyline antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Chlortetracyline in it. This value is compared to the standard curve and the Chlortetracyline concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue about 3ppb
Cross-reaction rate:
Chlortetracyline 100%
Tetracyclines 80%
Oxytetracyline 40%
Doxycycline 8%
Recovery rate:
Tissue 90±15%
3. Components
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;
2) Before the experiment, each experimental equipment must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.
Solution preparation before sample pre-treatment:
5.1 Tissue
Fold of dilution of the sample: 12
6. ELISA procedures
Instructions
1. Bring all reagents and micro-well strips to the room temperature (20-25 ℃).
2. Return all reagents to 2-8 ℃ immediately after use.
3 .The reproducibility of the ELISA analysis, to a large degree, depends on the consistency of plate washing. The correct operation of plate washing is the key point in the procedures of ELISA.
4. For the incubation at constant temperatures, all the samples and reagents must avoid light exposure, and each microplate should be sealed by the cover membrane.
Operation procedures
7. Result judgment
Quantitative determination
The mean values of the absorbance values is equivalent to the percentage of the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average (double wells) OD value of the sample or the standard solution
B0—the average OD value of the 0 ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solutions and the semilogarithm values of the Chlortetracyline standard solutions (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, thus finally obtaining Chlortetracyline concentration in the sample.
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com