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Product Details:
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Sensitivity: | 0.5 Ppb | Incubator Temperature: | 25℃ |
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Incubator Time: | 30min~15min | Sample Performance: | Tissue |
Shelf Life: | 12 Months When Properly Stored | MOQ: | 1 Kit |
High Light: | mqca elisa kit,mqca elisa test kit,food safety elisa kit |
Olaquindox metabolites (MQCA) ELISA Test Kit
Catalog No. LSY-10046
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of MQCA in the sample. The coupling antigens are pre-coated on the micro-well stripes. The MQCA in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-MQCA antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the MQCA in it. This value is compared to the standard curve and the MQCA concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.5 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
Tissue 1 ppb
Cross-reaction rate:
MQCA 100%
Olaquindox <0.1%
Recovery rate:
Tissue 85%±10%
3. Components
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
Sample preparation
1) Sample extract (Store at 2~8 ℃ for 1 month): Take 8.6ml concentrated HCl, add deionized water to 500ml, then add 10g NaCl to get the sample extract.
5.1 Livestock and poultry tissue (Chicken, duck, pork):
56℃ water bath;
Fold of dilution of the sample: 0.5
6. ELISA procedures
6.1 Instructions
6.2 Operation procedures
7. Result judgment
There are two methods to judge the results; the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the content of MQCA.
7.1 Qualitative determination
The concentration range (ng/mL) can be obtained from the comparison the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sample Ⅰ is 0.3, and that of the sample Ⅱ is 1.0, while those of the standard solutions are as the followings: 2.043 for 0 ppb, 1.716 for 0.5 ppb, 1.215 for 1.5 ppb, 0.74 for 4.5 ppb, 0.313 for 13.5 ppb and 0.155 for 40.5 ppb, accordingly the concentration range of the sampleⅠis 13.5 to 40.5ppb, and that of the sample Ⅱ is 1.5 to 4.5 ppb.
7.2 Quantitative determination
The mean values of the absorbance values is equivalent to the percentage of the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average (double wells) OD value of the sample or the standard solution
B0—the average OD value of the 0 ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solutions and the semilogarithm values of the MQCA standard solutions (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, thus finally obtaining MQCA concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software)
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.