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Product Details:
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Sensitivity: | 0.1 Ppb | Incubation Temperature: | 25℃ |
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Incubation Time: | 30min ~ 15min | Sample Performance: | Tissue,feed, Milk, Honey,urine, Milk Powder |
Shelf Life: | 12 Months When Properly Stored | Specifications: | 96 Wells/kit |
MOQ: | 1 Kit | ||
High Light: | food safety test kit,milk test kit,honey elisa kit |
Dexamethasone ELISA test Kit
Catalog No. LSY-10042
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Dexamethasone in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Dexamethasone in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Dexamethasone antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Dexamethasone in it. This value is compared to the standard curve and the Dexamethasone concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Incubation Temperature: 25℃
Incubation Time: 30min ~ 15min
Detection limit:
Tissue 0.1 ppb
Feed, milk, honey, urine 0.2 ppb
Milk powder 0.3 ppb
Cross-reaction rate:
Dexamethasone 100%
Recovery rate
Tissue, feed 90±25%
Milk powder 85±25%
Urine, milk, honey…………………………………………………………. 95±25%
3. Components
1 | Micro-well strips |
12 strips with 8 removable wells each |
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2 | 6× standard solution (1mL each) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | Enzyme conjugate | 7ml | red cap |
4 | Antibody working solution | 7ml | blue cap |
5 | Substrate A | 7ml | white cap |
6 | Substrate B | 7ml | black cap |
7 | Stop solution | 7ml | yellow cap |
8 | 20× concentrated washing buffer | 40ml | white cap |
9 | 5× concentrated redissolving solution | 50ml | transparent cap |
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;
2) Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination which interferes with the experimental results.
Solution preparation before sample pre-treatment
5.1 Tissue
Fold of dilution of the sample: 1
5.2 Feed
1. Weigh 1.0±0.05g homogenized feed sample into 50ml centrifuge tube, add 6ml ethyl acetate, shake for 3 min, centrifuge at above 4000 r/min at 15 ℃ for 10 min.
2. Take 3 ml clear organic phase into a dry container, blow to dry with nitrogen or air at 50-60℃.
Fold of dilution of the sample: 2
5.3 Milk powder
Fold of dilution of the sample: 3
5.4 Honey
Fold of dilution of the sample: 2
5.5 Urine
Fold of dilution of the sample: 2
5.6 Milk
1. Take 1.0ml milk sample into a 5ml centrifuge tube, add 2ml Acetonitrile and Ethyl acetate mixing solution, shake for 1min, centrifuge at above 4000 r/min at 15℃ for 10 min.
Fold of dilution of the sample: 2
6. ELISA procedures
6.1 Instructions
6.2 Operation procedures
7. Result judgment
There are two methods to judge the results; the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the content of Dexamethasone.
7.1 Qualitative determination
The concentration range (ng/mL) can be obtained from the comparison the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sampleⅠ is 0.3, and that of the sampleⅡ is 1.0, while those of the standard solutions are as the followings: 2.243 for 0 ppb, 1.816 for 0.1 ppb, 1.415for 0.3 ppb, 0.74 for 0.9 ppb, 0.313 for 2.7 ppb and 0.155 for 8.1 ppb, accordingly the concentration range of the sampleⅠis 2.7 to 8.1 ppb, and that of the sampleⅡ is 0.3 to 0.9 ppb.
7.2 Quantitative determination
The mean values of the absorbance values obtained for the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average OD value of the sample or the standard solution
B0—the average OD value of the 0ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solution and the semilogarithm values of the Dexamethasone standard solution (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, thus finally obtaining the Dexamethasone concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software) .
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on the box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.