Nitrofuran(AHD) rapid test strip

Nitrofuran(AHD) rapid test strip

Catalogue No.: LSY-20052

1. Brief

This product is used for testing Nitrofuran(AHD) residue in Aquatic products. The whole testing needs about 2h, suitable for all kinds of enterprises and inspection agencies.

The detection limit is 1.0ppb（ug/kg）

2. Components

Nitrofuran (AHD) colloidal gold rapid test strip

(Including tablet, gold label well, dropper, desiccant) 50 pieces/kit

3. Equipmentsrequired but not provided

Homogenizer, Micropipettes, nitrogen-drying device or air-drying device, water bath, centrifuge, balance (a reciprocal sensibility of 0.01 g);

4. Sample preparation

1) Take a certain quantity of fat-free tissue sample, cut it into pieces with scissors;

2) Weigh 2g shredded tissue sample into 50ml centrifuge tube, add 4ml deionized water, 0.5ml

1M HCl, 0.2ml 2-Nitrobenzaldehyde (C7H5NO3), mix fully for 3min;

3) Incubator at 60℃ water bath for 1h;

4) Take it out, add 5ml 0.1M K2HPO4, 0.4ml 1M NaOH, add 6ml Ethyl acetate, mix fully for 1min, centrifuge at 4000r/min for 5min;

5) Take 3ml upper layer liquid into 5ml centrifuge tube, blow to dry by air at 60℃.

6) Add 1ml Hexane to dissolve the dry residue, cup and shake for 1min, then add 0.5ml Nitrofuran redissolving solution, mix evenly, centrifuge at 4000r/min for 1min. (Or be static until obvious stratification );

7) Absorb 80ul down-layer solution to test.

5.Operation procedures

1) Read the Instruction, return the reagents to room temperature (20℃~30℃) before testing.

2) Take out the test strip, put it on desk flatly, as the following picture on the right. (Use it immediately after opening the package.)

3) Absorb80μLsolution into gold label well, use small dropper to blow and beat until the red material is completely dissolved, wait to react for 5min,

4) Use dropper absorb all sample from the well, vertically add it into sample collecting well (as following picture), timing after adding sample.

5) Read the result in 5-8 minutes, otherwise it is invalid.

Note: S means Sample Colleting Region

C means Control Line

T means Test Line

6.Test Result Interpretation

Negative Positive Invalid

6.1 Negative (—): T line appears (T line, near Sample Collecting Region), T line is darker or has the same color with C line, it means the AHD concentration is lower than detection limit or no AHD residue.

6.2 Positive (+): T line is lighter than C line or T line is invisible, it means the AHD concentration is higher than detection limit. The more lighter of T line compared with C line, the higher content of AHD in sample.

6.3 Invalid: C line is invisible, maybe it is the operation error or test strip is invalid. Read the Instruction, use new test strip to test again.

7.Precautions

1) Test at 20℃~30℃ environment.

2) Wear gloves when testing.

3) Do not eat any of the reagents.

4) Do not use test card out of expiry date.

5) Do not touch the white membrane surface in the middle of test card.

6) Do not repeat use the dropper, to avoid cross pollution.

8. Cross-reaction Rate

.

9.Storage and expiry date

Storage:Store at 4-30 ℃ in dark,no frozen.

Expiry date:12 months; date of production is on box.