LSY-10047 Nitromidazoly ELISA Test Kit Food testing ISO certificated

Nitromidazoly ELISA Test Kit

Catalog No. LSY-10047

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Nitromidazoly in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Nitromidazoly in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Nitromidazoly antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Nitromidazoly in it. This value is compared to the standard curve and the Nitromidazoly concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1ppb

Detection limit

Pork, Chicken....................................................................... about 0.5ppb

Honey ................................................................................ about 0.1ppb

Note: ppb= ng/ml or ng/g

Cross-reaction rate

Metronidazole.................................................................................. 100%

Hydroxyl metronidazole..................................................................... 47%

Hydroxymethyl dimetridazole............................................................. 53%

Tinidazole........................................................................................... 2%

Dimetridazole.................................................................................. 800%

Ornidazole.......................................................................................... 5%

Recovery rate

Tissue, Honey.............................................................................. 90±30%

3. Components

1. Micro-well strips: 12 strips with 8 removable wells each
2. 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb
3. Enzyme conjugate (12 mL)............................................................ 1 bottle
4. Antibody working solution (6mL)................................................... 1 bottle
5. Substrate A solution (6 mL)........................................................... 1 bottle
6. Substrate B solution (6 mL)........................................................... 1 bottle
7. Stop solution (6 mL)..................................................................... 1 bottle
8. 20× concentrated washing buffer (30 mL)...................................... 1 bottle
9. Redissolving solution (50 mL)....................................................... 1 bottle
10. Tissue sample treatment solutionⅠ(200ml deionized water to dissolve) 1 bottle