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Product Details:
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Sensitivity: | 0.1 Ppb | Incubator Temperature: | 25℃ |
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Incubator Time: | 30min~15min | Shelf Life: | 12 Months When Properly Stored |
MOQ: | 1 Kit | Sample Performance: | Tissue |
High Light: | Lincomycin elisa kit,antibiotic residue elisa kit,honey test kit |
Lincomycin ELISA Test Kit
Catalog No. LSY-10045
1. Principle
The test kit is based on the indirect competitive enzyme immunoassay for the detection of Lincomycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Lincomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Lincomycin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Lincomycin in it. This value is compared to the standard curve and the Lincomycin residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit
Tissue 0.4 ppb
Cross-reaction rate
Lincomycin 100%
Recovery rate
Tissue……………………………………………………………..……90%±35%
3. Components
10) 6× concentrated sample extraction B (50 mL) …………….…transparent cap
4. Materials required but not provided
5. Sample pre-treatment
Instructions (The following points must be dealt with before the pre-treatment)
Solution preparation before sample pre-treatment:
1 part 10× concentrated sample extraction A + 9 parts deionized water.
1 part 6× concentrated sample extraction B + 5 parts deionized water.
Samples preparation
5.1 Tissue (Chicken, Pork/liver, duck, shrimp, fish etc.)
Fold of dilution of sample: 4
6. ELISA procedures
Instructions
plate washing. The correct operation of plate washing is the key point in the procedures of ELISA.
Operation procedure
7. Result judgment
There are two methods to judge the results; the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with Lincomycin concentration in the sample.
7.1 Qualitative determination
The concentration range (ng/mL) can be obtained from comparing the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sample Ⅰ is 0.238, and that of the sample Ⅱ is 0.946, the OD value of standard solutions is: 1.845 for 0 ppb, 1.542 for 0.1 ppb, 1.130 for 0.3ppb, 0.635 for 0.9ppb, 0.326 for 2.7ppb, 0.156 for 8.1ppb, accordingly the concentration range of the sample Ⅰ is 2.7 to 8.1ppb, and that of the sample Ⅱ is 0.3 to 0.9ppb.
7.2 Quantitative determination
The mean values of the absorbance values obtained for the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average (double wells) OD value of the sample or the standard solution
B0—the average OD value of the 0ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solutions and the semilogarithm values of the Lincomycin standard solutions (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, finally obtaining the Lincomycin concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software)
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.
Remarks: If the vacuum package of microtiter plates has leakage, the microtiter plate is normal and effective, do not affect the experimental result. Please feel free to use.