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Product Details:
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Sensitivity: | 0.1 Ppb | Incubator Temperature: | 25℃ |
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Incubator Time: | 30min~15min | Sample Performance: | Honey, Tissue, Egg, Milk, Feed |
Shelf Lfie: | 12 Months When Properly Stored | Recovery Rate: | 90 ±30% |
MOQ: | 1 Box | ||
High Light: | elisa diagnostic kit,elisa immunoassay testing,Florfenicol assay kit |
Florfenicol ELISA Test Kit
Catalog No. LSY-10008
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in samples. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit:
Honey, tissue, egg 0.1ppb
Milk, feed 0.2ppb
Recovery rate:
Tissue, honey, milk, egg, feed 90 ±30%
Cross-reaction rate:
Florfenicol 100%
Florfenicol Amine 11%
theThiamphenicol < 0.1%
theChloramphenicol < 0.1%
3. Components
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
Solution preparation before sample pre-treatment:
Solution I Sample redissolving solution:
Dilute the 2× concentrated redissolving solution with deionized water at 1:1 (1 part 2× concentrated redissolving solution +1 part deionized water).
Solution Ⅱ Sample extracting solution:
Mix Acetonitrile and Ethyl acetate at 1:2 evenly (1 part Acetonitrile + 2 parts Ethyl acetate )
Samples preparation
a) Tissue (Chicken/liver, pork/liver, shrimp, fish etc.)
Fold of dilution of the sample: 1
b) Honey
1 Put 2±0.05 g honey sample into centrifugal tube, dissolved with 4 mL deionized water. Add 4 mL ethyl acetate, shake for 2 min. Centrifuge at above 4000 r/min at room temperature for 10 min.
2 Transfer 2ml supernatant into another centrifuge tube, blow to dry with nitrogen at 50-60 ℃. Add 1 mL of the sample redissolving solution to dissolve dry residue, mix for 30 seconds.
3 Take 50 µL for analysis.
Fold of dilution of the sample: 1
c) Milk
Fold of dilution of the sample: 2
d) Feed
Fold of dilution of the sample: 2
e) Egg
Fold of dilution of the sample: 1
(If the 3ml supernatant cannot be obtained during processing, the solution can be taken after repeated centrifugation, or 5ml can be taken out after adding 10ml of ethyl acetate, and the dilution can be maintained.)
6. ELISA procedures
6.1 Instructions
6.2 Test implementation
1 Take out all the necessary reagents from 2~8 ℃ environment, bring them to the room temperature (20-25 ℃) for at least 30 min, note that each liquid reagent must be shaken evenly before use.
7. Result judgment
There are two methods to judge the results; the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the content of Florfenicol.
7.1 Qualitative determination
The concentration range (ng/mL) can be obtained from comparing the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sampleⅠ is 0.3, and that of the sampleⅡ is 1.0, the OD value of standard solutions is: 2.243 for 0ppb, 1.816 for 0.1ppb, 1.415 for 0.2ppb, 0.74 for 0.4ppb, 0.313 for 0.8ppb, 0.155 for 1.6ppb, accordingly the concentration range of the sampleⅠ is 0.8 to 1.6, and that of the sampleⅡ is 0.2 to 0.4ppb.
7.2 Quantitative determination
The mean values of the absorbance values obtained for the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average (double wells) OD value of the sample or the standard solution
B0—the average OD value of the 0 ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solutions and the semilogarithm values of the Florfenicol standard solutions (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, thus finally obtaining the Florfenicol concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software).
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.