Methomyl Rapid Test Card pesticide residue rapid detection of testing Methomyl in vegetables and fruits qualitatively

Methomyl Rapid Test Card

Catalog No.: GN83AE1

1. Brief

This product is used for testing Methomyl in vegetables and fruits qualitatively. The whole test only needs 15 min, easy to operate with high sensitivity. It is suitable for rapid on-site and laboratory testing.

2. Detection limit: 0.2 mg/kg(ppm)

3. Application: vegetables and fruits

4. Date of manufacture and batch number: See bag and outer box for details.

5. Principle

The Methomyl rapid test card applies the principle of competitive inhibition immunochromatography. During the micro-well incubation and chromatography process, Methomyl in the sample binds to the colloidal gold-labeled specific Methomyl monoclonal antibody, inhibiting the binding of the antibody to the Methomyl-BSA conjugate on the NC membrane testing line, thereby causing a change in the depth of the testing line color.

6. Components

7. Auxiliary equipment

Electronic balance (with an accuracy of 0.1g or above), scissors, single-channel pipettes (100-1000μl, 20-200μl), oscillator

8. Sample Pretreatment

Weigh 2g±0.1g of the vegetable/fruit sample into a 50ml centrifuge tube (cut leafy vegetables into pieces about 1cm square, and take cross-sectional samples or their skins for tubers), add 3ml of the Sample extraction buffer, shake by hand to mix well (vigorous shaking is prohibited) for 2 minutes, and let it stand to obtain the sample solution.

9. Operation procedures

1) Take out the test card, micro-well and dropper from the aluminum foil bag and use them within one hour.

2). Before testing different types of samples, users need to dilute the sample solution. For details, please refer to the following dilution list.

3). Use a dropper to draw 4 drops (about 100 uL) of the diluted solution into the micro-well. Gently pipette for 30 seconds to completely dissolve the red substance at the bottom of the microwell. Let it stand horizontally and react for 2 minutes. Then draw the red solution from the microwell, gently pipette for 10 seconds, and add it all to the sample loading hole (S) of the test card. Start timing after adding the sample.

4). Let it stand for reaction. After 5 minutes, determine the result based on the schematic diagram. Other interpretation times are invalid.

Dilution list

10. Test Result Interpretation

1) Negative: the color of T line is darker than C line or T line has same color with C line; it means there is no Methomyl residue or the residue is lower than detection limit.

2) Positive: T line is obviously lighter than C line or T line is invisible; it means the Methomyl residue is equal to or higher than detection limit.

3) Invalid test: If C line does not show which means the test cassette is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the local distributor.

11. Precautions

1). The test card is for single use at room temperature. Never use expired test cards.

2). Disposable droppers must not be reused to avoid cross-contamination.

3). Try not to touch the white membrane surface in the center of the test card during use. Avoid direct sunlight and direct fan blowing.

4). Tap water, distilled water or deionized water cannot be used as negative controls.

5). If a positive result is detected, it is recommended to retest with this card once.

6). If you encounter any problems during the test, please contact our company.

12. Specificity

This product has no cross-reaction with other types of pesticides such as Carbofuran, carbendazim, and chlorpyrifos.

13. Storage and expiry date

Storage: Store at 2-30℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months

14. Safety Instructions

This product does not contain carcinogenic, highly toxic, flammable, explosive or strongly corrosive reagents.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-18165709090/86-755-28438788 Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com