PA94A\01 Newcastle disease virus (NDV) nucleic acid test kit (Fluorescent RT-PCR) a one-step qRT-PCR nucleic acid test kit designed for the auxiliary detection of NDV nucleic acid in samples.

Newcastle disease virus (NDV) nucleic acid test kit

(Fluorescent RT-PCR)

[Product Overview]

This product is a one-step qRT-PCR nucleic acid test kit designed for the auxiliary detection of NDV nucleic acid in samples. Specific primers and a specific fluorescent probe targeting the conserved region of the NDV gene have been developed; the probe is labeled with the FAM fluorescent dye. When combined with the qRT-PCR reaction mix, this kit enables rapid detection of NDV nucleic acid using a fluorescent quantitative PCR instrument.

The kit includes a positive control and a negative control. The positive control consists of a plasmid containing the amplification fragment, used to assess the validity of the assay; the negative control is used to detect the presence of contamination in the reaction system.

[Product Composition]

[Storage Conditions]

Store at-20 °C, protected from light; shelf life: 12 months.

[Applicable Instruments]

Real-time fluorescent PCR instruments, such as the ABI 7500, Bio-Rad CFX96, and SLAN®-96P.

[Sample Requirements]

• Customers are required to provide their own reagents: normal saline.
• Collected serum, blister skin, blister fluid and other sample should be stored and transported at 2–8 °C and promptly submitted for laboratory testing. Alternatively, the samples may be preserved in viral transport medium and then sent to the laboratory for analysis. Takes tissues such as larynx, trachea, muscle, or lungs for death or culling of poultry; Use a cotton swab to collect respiratory secretions or excreta for live poultry, and place them in the virus sample preservation solution. Store and transport at 2-8 ℃, and send for inspection as soon as possible.

Note: Samples submitted for testing must be fresh; repeated freeze-thaw cycles are strictly prohibited.

• It is recommended to use a commercial viral nucleic acid extraction kit for RNA extraction and purification; the extracted RNA can then be directly used for detection. If samples are not analyzed immediately after extraction, they may be stored at-80 °C for later use; however, repeated freeze-thaw cycles should be avoided.