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PA34A\01 Bovine Viral Diarrhea Virus (BVDV) nucleic acid test kit (Fluorescent RT-PCR) one-step qRT-PCR nucleic acid test kit designed for the auxiliary detection of BVDV nucleic acid in samples.
Product Details:
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Detailed Product Description
| CV: | CV<3% | Specificity: | 100% |
|---|---|---|---|
| Shelf Life: | 12 Months | Sample Typesurfaces; Wipes For Animal Fur Are Placed In 50% Glycerol Physiological Saline Solution;: | Samples Including Serum, Tissue Samples, Nasal Swabs, Semen, Milk |
| Packing: | 50T/kit |
Bovine Viral Diarrhea Virus (BVDV) nucleic acid test kit
(Fluorescent RT-PCR)
| Catalog No. | Specifications | Storage | Transport | Version number |
| PA34A 1 | 50 T | -20℃ | low temperature | V1 |
[Product Overview]
This product is a one-step qRT-PCR nucleic acid test kit designed for the auxiliary detection of BVDV nucleic acid in samples. Specific primers and a specific fluorescent probe targeting the conserved region of the BVDV gene have been developed; the probe is labeled with the FAM fluorescent dye. When combined with the qRT-PCR reaction mix, this kit enables rapid detection of BVDV nucleic acid using a fluorescent quantitative PCR instrument.
The kit includes a positive control and a negative control. The positive control consists of a plasmid containing the amplification fragment, used to assess the validity of the assay; the negative control is used to detect the presence of contamination in the reaction system.
[Product Composition]
| Code | Component | PA30A 1 |
| 1 | One-step qRT-PCR enzyme mix | 80 μL |
| 2 | BVDV qRT-PCR amplification reaction mix | 1250 μL |
| 3 | Positive control | 100 μL |
| 4 | Negative control | 100 μL |
[Storage Conditions]
Store at-20 °C, protected from light; shelf life: 12 months.
[Applicable Instruments]
Real-time fluorescent PCR instruments, such as the ABI 7500, Bio-Rad CFX96, and SLAN®-96P.
[Sample Requirements]
- Customers are required to provide their own reagents: normal saline.
- Collected serum, tissue samples, nasal swabs, semen, milk and other sample should be stored and transported at 2–8 °C and promptly submitted for laboratory testing. Alternatively, the samples may be preserved in viral transport medium and then sent to the laboratory for analysis.
Note: Samples submitted for testing must be fresh; repeated freeze-thaw cycles are strictly prohibited.
- It is recommended to use a commercial viral nucleic acid extraction kit for RNA extraction and purification; the extracted RNA can then be directly used for detection. If samples are not analyzed immediately after extraction, they may be stored at-80 °C for later use; however, repeated freeze-thaw cycles should be avoided.
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