Rapid Test Kits for detecting Tetracyclines antibiotic residue in eggs sample detection limit 10-20ppb

Tetracyclines rapid test strip (Egg)

Catalog No. LSY-20046G2d

1. Brief

This product is used for testing Tetracyclines in egg, duck egg etc. sample qualitatively. The whole test need 10min. Easy to operate, high sensitivity.

2. Detection limit

3. Contents

4. Principle

The colloidal gold immuno-chromatography was used to detect the Tetracyclines residue in the samples. The binding of Tetracyclines to the specific monoclonal antibody labeled with colloidal gold inhibited the binding of the antibody to the conjugates of Tetracyclines-BSA on the NC membrane Testing line, which resulted in the color depth variation of the testing line.

5. Testing equipment required

Instruments: balance, micropipette, oscillator (optional), homogenizer

Consumables: 15/50ml centrifuge tube, disposable tips.

6. Sample buffer: Dilute 10X concentrated sample buffer with deionized water at 1:9 to get sample buffer. (for example: 1 part of 10X concentrated sample buffer + 9 parts of deionized water)

7. Sample preparation

Eggs: take 1g fresh mixed well egg sample, add 4ml sample buffer, shake by hand or oscillator strongly for 1min, ready to test.

8. Operation procedures

8.1 Read the instruction carefully before use. Return test strips and sample into room temperature.

8.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

8.3 Take 200ul of the test samples into the microwells, then slowly aspirate for 5 times, mix the sample with the reagent in the microwells completely, until no solid observed by eyes (this is a very important step).

8.4 Incubate for 5min at room temperature (20-40℃), then insert the test strips into the microwells with the "MAX" end fully dipped in to the mixture solution.

8.5 After immersing the test strip in the micro-well for 5 minutes, take out the strip, interpret the results according to the schematic diagram. Please interpret the results within 1 minutes, otherwise interpretation will be invalid.

9. Test Result Interpretation

9.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Tetracyclines residue in sample or the residue is lower than detection limit.

9.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Tetracyclines residue is equal to or higher than detection limit.

9.3 Invalidation: C line isn’t seen wine red. It means the test strip is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

10. Precautions

1) The test strips can be used only once at room temperature, do not use test strips out of expiry date.

2) Please seal the bottle after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.

3) Do not touch the white membrane surface in the middle of test strips, avoid sunlight and fan blowing directly.

4) The egg sample must be a uniform liquid, without clumping, souring, or precipitation.

5) Use the test strips testing again for positive results.

6) Please contact the supplier for any questions.

7) The result is for reference only, for confirmation, please refer to the relevant national standard method.

11. Safety performance and garbage disposal

This product's test strips and packaging are non-toxic and harmless, and should be disposed of as dry waste after use.

12. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com