Rapid Test Kits for detecting Gentamicin antibiotic residue in eggs sample detection limit 20ppb

Gentamicin rapid test strip (Egg)

Catalog No.: LSY-20043G4

1. Brief

This product is used for testing Gentamicin residue in egg, duck eggs etc. sample qualitatively. The total test time only needs 10 min. Easy to operate, high sensitivity.

2. Detection limit: 20μg/kg(ppb)

3. Specifications: 96tests/box

4. Principle

The Gentamicin rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, Gentamicin in the sample combined with Gentamicin specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Gentamicin-BSA conjugate on Test line of NC membrane, lead to the color change of Test line.

5. Contents

6. Materials needed but not provided.

Instruments: electronic balance, micropipette, vortex oscillator, homogenizer.

Consumables: 15/50ml centrifuge tube, disposable tips.

7. Sample preparation

Dilute 10X Sample extraction buffer with deionized water at 10 times before use. (1 part of 10X Sample extraction A + 9 parts of deionized water)

Sample preparation

Take 1g of fresh and evenly mixed egg sample, add 4ml of sample extraction buffer, vigorously shake by hand or vortex shaker for 1 minute, ready for testing.

8. Operation procedures

8.1 Read the instruction carefully before use. Return test strips and sample into room temperature.

8.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

8.3 Take 200µl of the test samples into the micro-wells, then repeatedly pump and suck for many times, mix the sample with the reagent in the micro-wells completely until no solid judge by eyes (this is a very important step).

8.4 Incubate for 5min at room temperature (20-40℃), then insert the test strips into the micro-wells with the "MAX" end fully dipped in to the mixture solution.

8.5 After immersing the strips into the microwell for 5min, take out the strip, then read the result within 1min, it is invalid in other time.

9. Test Result Interpretation

9.1 Negative: C line appears, T line is darker than C line, or T line has same color with C line. It means there is no Gentamicin residue in sample or the residue is lower than detection limit.

9.2 Positive: C line appears, T line is obviously lighter than C line or T line is invisible. It means the Gentamicin residue is equal to or higher than detection limit.

9.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

10. Precautions

1). The test strip is disposable at room temperature; Do not use expired test strips.

2). Disposable tips cannot be reused to avoid cross contamination.

3). Try not to touch the white film surface in the center of the test strip during use; Avoid direct sunlight and direct fan blowing.

4). The egg sample must be a uniform liquid, without clumping, souring, or precipitation.

5). Tap water, distilled water, deionized water, or sample extracts cannot be used as negative controls.

6). If a positive result appears, it is recommended to retest with this test strip.

7). Due to differences in samples, some detection lines may appear lighter or grayish in color, but as long as a red band appears, it can be determined.

8). If you encounter any problems during the experiment, please contact our company.

11. Safety performance and garbage disposal

This product's test strips and packaging are non-toxic and harmless, and should be disposed of as dry waste after use.

12. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788 Fax 86-755-28938800

Email: info@lsybt.com

Website: www.lsybt.com