Green Spring theChloramphenicol(CAP) rapid test dipsticks for honey safety detection

TheChloramphenicol(CAP) rapid test dipsticks (Honey)

Catalog No. LSY-20004

1. Brief

This product is used for testing theChloramphenicol in honey sample qualitatively. Easy to operate, high sensitivity.

2. Detection limit

Honey: 0.1ng/ml (0.1ppb)

3. Contents

(1) 8 microwells and 8 dipsticks in one bottle 12 bottles / kit.

(2) Instruction 1 piece

(3) 10X concentrated sample buffer 1 bottle

4. Principle

TheChloramphenicol rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, theChloramphenicol in the sample combined with theChloramphenicol specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and theChloramphenicol-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no theChloramphenicol residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is theChloramphenicol residue in sample, C line will appear, it means the test is valid.

5. Materials needed but not provided.

Equipment: homogenizer, nitrogen-drying device (or hair dryer), vortex, oscillator (optional), centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g).

Reagents: 50ml centrifuge tube, tips, ethyl acetate.

6. Sample preparation

1) Sample buffer: Dilute 10X concentrated sample buffer with deionized water at 1:9 to get sample buffer. (1.5ml 10X concentrated sample buffer + 13.5ml deionized water)

2) Honey: Take 3±0.05g honey sample into 50ml centrifuge tube, add 3ml deionized water, then add 6ml ethyl acetate, shake by hand or oscillator for 2min, centrifuge at 4000r/min for 10min, take 4ml supernatant into another centrifuge tube, blow to dry at 56℃ by Nitrogen or air (or use hair dryer). Add 0.5ml sample buffer, shake for 30s, dissolve the solid on wall and bottom of tube fully, the solution is ready to test.

7. Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

7.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

7.3 Take 80ul of the test samples into the microwells, then repeatedly absorb for many times, mix the sample with the reagent in the microwells completely (this is a very important step).

7.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.

7.5 Incubate for 3-5min at room temperature again, read the result, it is invalid in other time.

8. Test Result Interpretation

8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no theChloramphenicol residue in sample or the residue is lower than detection limit.

8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means theChloramphenicol residue is equal to or higher than detection limit.

8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

9. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

3) Use the test dipsticks testing again for positive results.

4) Please contact the supplier for any questions.

10. Specificity

Test 500ppb Sulfonamides, quinolones and tetracyclines and other drugs. All the results are negative.

11. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788 Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com

Test procedure