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Product Details:
Payment & Shipping Terms:
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Sensitivity: | 0.05ppb | Incubator Temperature: | 25℃ |
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Incubator Time: | 30min~15min | Shelf Life: | 12 Months When Properly Stored |
Specifications: | 96 Wells/kit | MOQ: | 1 Kit |
Sample Performance: | Honey |
Sulfamethazine (SM2) ELISA Kit for Honey
Catalog No.: EF58BJ1
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Sulfamethazine (SM2) residue in honey sample. The coupling antigens are pre-coated on the micro-well stripes. The Sulfamethazine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Sulfamethazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Sulfamethazine in the sample. This value is compared to the standard curve and the Sulfamethazine concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05 ppb
Incubator temperature: 25℃
Incubator time: 30min~15min
Detection limit
Honey........................................................................................... 0.5 ppb
Cross-reaction rate
Sulfamethazine(SM2)………………………………………………………. 100%
Sulfamerazine (SM1)........................................................................ <1%
Sulfadimethoxine(SDM)…………………………………………………… <1%
Sulfadiazine (SD or SDZ)................................................................. <1%
Sulfamonomethoxine (SMM) …………………………………………….. <1%
Sulfamethoxydiazine(SMD)............................................................... <1%
Sulfamethoxazole (SMZ)........................................................ ….. <1%
Sulfaguanidine(SG) .............................................................. ….. <1%
Sulfathiazole (ST)............................................................................ <1%
Sulfaquinoxaline(SQX)………………………………………………………<1%
Sulfamethoxypyridazine (SMP)……………………………………………<1%
Recovery rate
Honey...................................................................................... 90% ±30%
3. Components
1 | Micro-well strips |
12 strips with 8 removable wells each |
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2 | 6× standard solution (1mL each) | 0ppb | 0.05ppb |
0.15ppb | 0.45ppb | ||
1.35ppb | 4.05ppb | ||
3 | High concentration standard | 1ml | 100 ppb |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A | 7ml | white cap |
7 | Substrate B | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× concentrated washing buffer | 15ml | white cap |
10 | 20× Extracting buffer | 50ml*2 | transparent cap |
4. Materials required but not provided
5. Sample pre-treatment
Instructions (The following points must be dealt with before the pre-treatment)
Solution preparation before sample pre-treatment:
Extracting buffer: 1 part of 20× Extracting buffer + 19 parts of deionized water, mix it evenly;
5.1 Honey
A. Method one
1) Weigh 1.0±0.05 g of the homogenized honey sample into 50 ml centrifuge tube, add 19 ml Extracting buffer, vortex for 3 min;
Fold of dilution of the sample: 20
B. Method two
1) Weigh 1.0±0.05 g of the homogenized honey sample into 15ml/50ml centrifuge tube, add 4 ml Extracting buffer, vortex for 3 min;
2) Take 100µl up-layer liquid, add 300µl Extracting buffer, mix it evenly;
Fold of dilution of the sample: 20
6. ELISA procedures
6.1 Instructions
6.2 Operation procedures
7. Result judgment
There are two methods to judge the results; the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the content of Sulfamethazine in the sample.
7.1 Qualitative determination
The concentration range (ng/mL) obtained from the comparison the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sample Ⅰ is 0.3, and that of the sample Ⅱ is 1.0, the OD value of standard solutions is: 2.243 for 0ppb, 1.816 for 0.05ppb, 1.415 for 0.15ppb, 0.74 for 0.45ppb, 0.313 for 1.35ppb, 0.155 for 4.05ppb, accordingly the concentration range of the sample Ⅰ is 1.35ppb~4.05ppb, and that of the sample Ⅱ is 0.15ppb~0.45ppb. (multiplied by the corresponding dilution fold)
7.2 Quantitative determination
The mean values of the absorbance values is equivalent to the percentage of the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average (double wells) OD value of the sample or the standard solution
B0—the average OD value of the 0ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solutions and the semilogarithm values of the Sulfamethazine standard solutions (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, finally obtaining the Sulfamethazine concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software)
8. Precautions
8. The optimum reaction temperature is 25 ℃, and too high or too low temperatures will result in the changes in the detecting sensitivity and OD values.
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 1 year; date of production is on box.
Note: If the Vacuum package of microplate has leakage, it is still valid to use, do not affect the test result, be relax to use.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com