LSY-20046 Tetracycline diagnostic rapid test for seafood 96 tests/kit

Tetracyclines rapid test dipstick (Tissue)

Catalog No. LSY-20046

1. Brief

This product is used for testing Tetracyclines residue in chicken, fish and shrimp sample qualitatively. The total test time need 15-30 min. Easy to operate, high sensitivity.

2. Detection limit

10-15 ppb(ug/kg)

• Specifications: 8 dipsticks/bottle, 12 bottles/box

4. Principle

The Tetracyclines rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Tetracyclines in the sample combined with Tetracyclines specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Tetracyclines -BSA conjugate on Test line of NC membrane, lead to the color change of Test line.

When the sample has no Tetracyclines residue or concentration lower than detection limit, T line is darker than C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line, T line has no color or T line has same color with C line. No matter whether there is Tetracyclines residue in sample, C line will appear, it means the test is valid.

5. Contents

6. Sample preparation

Sample extraction liquid A: Dilute 10X Sample extraction liquid A with deionized water at 10 times before use. (1 part 10X Sample extraction liquid A + 9 parts deionized water)

Sample preparation of chicken, fish

Weigh 3±0.05g tissue, add 3ml diluted Sample extraction liquid A, shake for 3min; then add 300ul Sample extraction liquid B, shake for 3min; Centrifuge at 4000rpm for 10min, take the up-layer clear liquid to test.

Sample preparation of shrimp

Weigh 3±0.05g tissue, add 3ml diluted Sample extraction liquid A, shake for 3min; then add 240ul 1M Sample extraction liquid B, shake for 3min; Centrifuge at 4000rpm for 10min, take the up-layer clear liquid to test.

7. Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

7.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

7.3 Take 200ul of the test samples into the micro-wells, then repeatedly absorb for many times, mix the sample with the reagent in the micro-wells completely until no solid judge by eyes (this is a very important step).

7.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.

7.5 Incubate for 5min at room temperature again, read the result, it is invalid in other time.

8. Test Result Interpretation

9. Precautions

1) The test strip can be used only once at room temperature, do not use test strip out of expiry date.

2) Cup the bottle immediately after taking our the reagents. If the 8 Microc-wells can not be used at one time, Cup the rest one immediately and store in sealed.

3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.

4) Re-test for positive result.

5) Please contact the supplier for any questions.

6) The result is for reference only, to confirm, please refer to national standard.

10. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788 Fax 86-755-28938800

Email: info@lsybt.com Website: www.lsybt.com