Veterinary Drug residue detection Clonidine ELISA test Kit

Green Spring Clonidine ELISA Test Kit

Catalog No. LSY-10039

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Clonidine in the sample of Tissue,feed etc.The coupling antibodies are pre-coated on the micro-well stripes. The Clonidinein the sample and the coupling antibodies pre-coated on the micro-well stripes compete forthe anti-Clonidine antigens, add TMB substrate for coloration. The higher Clonidine in the sample, the less ELISA antigen combined at the solid phase, the lighter of the coloration. The optical density (OD) value of the sample has a negative correlation with the Clonidine in it. This value is compared to the standard curve and the Clonidine concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05 ppb

Incubation Temperature: 25℃

Incubation Time:30min—(10-15)min

Detection limit

Urine··························································································· 0.05 ppb

Tissue (Method 1)········································································· 0.2 ppb

Tissue (Method 2)········································································· 0.1 ppb

Feed ···························································································· 0.5ppb

Cross-reaction rate

Clonidine························································································· 100%

Romifidine····················································································· ﹤44%

Tizanidine························································································· ﹤8%

Xylazine······················································································ ﹤0.03%

Recovery rate

Urine···························································································· 90±10%

Tissue·························································································· 90±15%

Feed···························································································· 90±15%

3. Components

1) Micro-well strips: 12 strips with 8 removable wells each

2) 6× standard solution (1 mL each): 0 ppb, 0.05 ppb, 0.15ppb, 0.45 ppb, 1.35 ppb and 4.05 ppb

3) Enzyme lyophilized powder (2 tube)··············································· red cap

4) Enzyme diluent solution (12 mL)··················································· blue cap

5) Substrate A solution (7 mL)························································· white cap

6) Substrate B solution (7 mL)························································ black cap

7) Stop solution (7 mL)································································· yellow cap

8) 20× concentrated washing buffer (40 mL)···································· white cap