Deoxynivalenol(DON) rapid test card used for testing Deoxynivalenol in grain, feed qualitatively

Deoxynivalenol(DON) rapid test card

Catalog No.: LSY-20084E2K

1. Brief

This product is used for testing Deoxynivalenol in grain, feed qualitatively, the whole test procedure only need 10-15min, easy to operate with high sensitivity.

2. Specifications: 40tests/box

3. Contents

4. Application: Grain, feed

5.Detection limit: 1000~5000 μg/kg(ppb)

6. Date of manufacture and batch number: See bag and outer box for details.

7. Principle
The Deoxynivalenol(DON) rapid test card is based on competitive inhibition immuno-chromatographic principle. In the flow process, DON in the sample combined with DON specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and DON-BSA conjugate on Test line of NC membrane, lead to the color change of Test line.
 
8.Required equipment and consumables
Instruments: Electronic balance, centrifuge, micropipette, vortex mixer, homogenizer.
Consumables: 50ml centrifuge tubes, 2mL or 1.5mL centrifuge tube, disposable pipette tips, ethanol (analytical grade), purified water.
 
9.Pre-treatment requires liquid configuration
Extraction solution: 50% ethanol-water solution
Preparation method for 50% ethanol-water solution: 50mL of ethanol (analytical grade) + 50mL of pure water
 

10.Sample preparation

Weigh 5.0±0.05g homogeneous sample (passing through 20 mesh screen) into a 50mL centrifuge tube, add 20mL of Extraction solution into the centrifuge tube, fully shake for 3min, centrifuge at 4000r/min for 5min or static layering to obtain the supernatant.

Add DON sample dilution and supernatant according to the detection limit like below method:

1) 1000 μg/kg detection limit

Take 1000 μL DON sample dilution, add into 2mL or 1.5mL centrifuge tube, then add 60 μL supernatant, mix well and wait for test.

2) 3000 μg/kg detection limit

Take 1000 μL DON sample dilution, add into 2mL or 1.5mL centrifuge tube, then add 20 μL supernatant, mix well and wait for test.

3) 5000 μg/kg detection limit

Take 2000 μL DON sample dilution, add into 2mL or 1.5mL centrifuge tube, then add 23 μL supernatant, mix well and wait for test.

 
11. Operation procedures
1). Please read the instructions carefully before use. Allow this test card and the sample to reach room temperature before use.
2). Take out the required number of micro-wells and test card and make proper marks. Please use them as soon as possible within 1 hour. Seal the remaining reagents immediately to prevent moisture.
3). Use a micropipette to aspirate 100µl of the sample into a micro-well, gently pipette up and down to fully mix it with the lyophilized powder in the micro-well (this step is important).
4).Place the test card flatly and pipette all liquid from the micro-well and dispense it into the sample well marked “S” on test card.
5). Wait for 5 minutes, then observe the result, interpret the result within 1 minute according to the schematic diagram. Interpretation at other times is invalid.
 
12. Test Result Interpretation
12.1 Negative: T line is darker than or has same color with C line. It means there is no DON residue in sample or the residue is lower than detection limit.
12.2 Positive: T line is obviously lighter than T line or T line is invisible. It means the DON residue is higher than or equal to detection limit.
12.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact us.
 

 
13. Precautions
1). The test card is intended for single use at room temperature; do not use expired test strips. When opening the micro-well, please gently peel off the sealing film. Do not peel it off quickly with force, as this may cause powder to fly out.
2). Disposable pipette tips cannot be reused to avoid cross-contamination.
3). During use, try not to touch the white film surface in the center of the test card; avoid direct sunlight and direct fan blows.
4). Tap water, distilled water, or deionized water cannot be used as negative controls.
5). In case of a positive result, it is recommended to conduct a retest using a new test card.
6). Due to the complexity of the finished feed formula, it may contain interfering substances; there may be slight differences in the results between negative spiked samples and real samples due to different toxin binding modes.
7). This method is a screening method. In case of positive samples or suspected positive samples, please use a confirmatory method for confirmation.
8). If you encounter any issues during the test, please contact our company.
 
14. Safety performance and waste disposal
The test cards and their packaging of this product are non-toxic and harmless, and can be disposed of as dry garbage after use.
 
15. Storage and expiry date
Storage: Store at 2-30 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.
 
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com