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Product Details:
Payment & Shipping Terms:
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Packing: | 96tests/kit | Shelf Life: | 12 Months When Properly Stored |
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Sample Performance: | Fresh Milk, Pasteurized Milk, Pure Cow/goat Milk, Whole Milk Powder | Detection Limit: | 10ppb |
Fipronil Rapid Test Strips for milk
Catalog No.: GN18AG2
1. Brief
This product is used for testing Fipronil in dairy products qualitatively. The whole test only needs 10 min, easy to operate with high sensitivity.
2. Packing: 96tests/box
3. Components
1) 12 tubes: each tube includes 8 test strips and 8 micro-wells red solid reagent.
2) Manual: 1 piece.
4. Application
Dairy products: fresh milk, pasteurized milk, pure cow/goat milk, whole milk powder (dissolve 1g of milk powder in 9ml of pure water and mix thoroughly)
5. Detection limit
Test name | Detection limitμg/kg(ppb) |
Fipronil | 10 |
6. Date of manufacture and batch number: See bag and outer box for details.
7. Principle
The colloidal gold immuno-chromatography was used to detect the Fipronil residue in the samples. The binding of Fipronil to the specific monoclonal antibody labeled with colloidal gold inhibited the binding of the antibody to the conjugates of Fipronil-BSA on the NC membrane Testing line, which resulted in the color depth variation of the testing line.
8. Testing equipment required
Instruments: Micropipette, 1 incubator, 1 Reader (optional), timer.
Consumables: disposable tips
9. Operation procedures
1) Return the test strip and sample to room temperature before use.
2) Take out tube, open it and take out the required number of micro-wells and test strips, and mark them properly. Please use it as soon as possible within 1 hour. After taking out the reagent, please immediately cover the remaining micro-wells and strips lid to prevent the remaining reagent from getting damp.
3) Use a micropipette to pipette 200 µl of the sample into the micro-well, slowly aspirate 5 times until the mixture is uniform, and observe with the naked eye that there is no solid substance.
4) After incubating with a incubator (40±2℃) for 5 minutes, insert the test strip into the micro-well (with the MAX line end facing downwards, allowing it to be fully immersed in the solution).
4) After immersing the test strip in the micro-well for 5 minutes, interpret the results according to the schematic diagram. Please interpret the results within 1 minutes, otherwise interpretation will be invalid.
10. Test Result Interpretation
Color depth comparison of T line and C line | Result judgment | Result analysis |
T line > C line | Negative | Fipronil residue is lower than detection limit in sample |
T line = C line | Weak Positive | Fipronil residue is equal to detection limit in sample |
T line < C line or T line is invisible | Positive | Fipronil residue is higher than detection limit in sample |
C line is invisible | Invalid | The test dipstick is invalid, test again |
11. Precautions
1) The test strip can be used only once at room temperature, do not use test strips out of expiry date.
2) Please seal the tube after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to tube and sealed.
3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.
4) Milk samples should be fully liquid without any agglomeration and deposition.
5) Use the test strips testing again for positive results.
6) Milk samples with high fat content may have slower chromatography speed. It is recommended to extend the reaction time for the second step by 1 minute.
7) The results is for reference only, for confirmation, please refer to the relevant national standard methods.
8) Please contact the supplier for any questions.
12. Safety performance and garbage disposal
This product's test strips and packaging are non-toxic and harmless. After use, they should be disposed of as dry waste.
13. Storage and expiry date
Storage: Store at 2-8℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788 Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com