Green Spring Canine heartworm antigen lateral flow rapid test kit ISO9001 certificate

Canine heartworm antigen rapid test kit

Catalog No. : CK11

1. Principle

The rapid test kit uses immunochromatographic technology to qualitatively detect heartworm antigens in canine serum, plasma, or whole blood. After the sample is added to the sample well, it moves along the chromatographic membrane together with the colloidal gold-labeled anti-HW monoclonal antibody. If the HW antigen is present in the sample, it will bind to the antibody on the detection line and display a wine red color. No color reaction if HW antigen is not present in the sample

2. Components

3. Test procedure

1) Collect whole blood, separate serum or plasma (centrifuge at 2000-3000 r/min for 5~15min or separate out naturally at 4℃ overnight), or take whole blood with anticoagulants as sample. Take whole blood without anticoagulants as sample is also OK, but must test it immediately.

2) Open the package, take out test card, put it on clean, flat desk.

3) Use dropper to drop 1 drop (about 40ul) into the well mark “S” , then drop 3 drops of sample buffer into the well. At this time, you will see wine-red liquid flowing through the observation window. If the liquid does not flow, you can lightly press the area between the "sample hole" and the "observation hole" to help the liquid release.

4) Judge the result at room temperature in 5-10min, it is invalid beyond 10min.

4. Results judgment

1) Positive: control line and test line are both seen wine red;

2) Negative: only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red.

5. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

6. Notice

1) Do not use test card out of date and bad package;

2) Store at room temperature (< 30℃) is OK. If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.

3) Do not touch the white membrane surface in the middle of test card.

4) To ensure the test quality, take fresh, unpolluted sample. If the sample has a large amount of blood lipids, it will interfere with the detection. In this regard, after centrifugation, avoid the oil layer and take the serum for detection

5) If the serum can not be tested immediately, store at 4℃ for short time (in 48hours), store at below -20℃ for long-tern storage.

6) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.

7) Treat the test card and sample as pollutant according to local regulations.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com