Canine Influenza Virus(CIV) Ag Rapid Test card (colloidal gold method) to detect samples in the nasal cavity or phary

Canine Influenza Virus(CIV) Ag Rapid Test card

Catalog No. : LSY-20092

1. Brief

Canine influenza is also caused by specific viruses of the influenza virus genus. But there are significant differences with human influenza viruses and avian influenza viruses. The Canine Influenza Virus(CIV) Ag Rapid Test card (colloidal gold method) uses rapid immunochromatography technology to detect CIV antigens in nasal or pharyngeal swabs. After adding sample, if there is CIV antigen in the sample, then it binds to the colloidal gold labeled monoclonal antibody against CIV, forming an antigen-antibody complex that moves along the chromatographic membrane, and then binds to the antibody in the testing line area (T), displaying a wine red color; if there is no CIV antigen, then no color reaction.

2. Components

3. Test Procedure

1). Using a cotton swab to collect samples in the nasal cavity or pharynx. The swab should be inserted into the nasal cavity or pharynx and repeatedly rotated and wiped on the inner wall.

2). Insert the swab into the tube with sample buffer immediately, rotate the swab strongly on tube well for at least 10 times and mix the solution evenly, making the sample been dissolved in sample buffer fully. Press the swab on tube well above the liquid level, making the liquid been pressed out fully, discard swab.

3). Take out test card from sealed bag. Put it on flat surface, use dropper to draw upper-layer bright liquid, drop 5 drops(do not over adding) slowly, one by one, to the sample hole with “S” mark.

4). Put the test card at room temperature for 10-15min, read the result, the result is invalid after 15 minutes.

4. Result judgment

1) Positive: Both on T Line and C line being seen purplish-red color reaction.

2) Negative: no color reaction on Test Line (T Line),only on Control Line(C Line) being seen purplish-red color reaction.

3) Invalid: In the observation window, there are no color lines in the control line area (C) and the testing line area (T); Or only color lines appear in the testing line area (T)., it means the operation is wrong or the test card is invalid, test again.

5. Precautions

1). Products that have expired or have damaged aluminum foil packaging bags cannot be used.

2). Please use the accompanying sample buffer. Do not mix Sample buffer between different products or Sample buffer from different batches of the same product.

3). The test card must be used within 1 hour after removal.

4). The collected sample should be tested immediately, avoid long time storage.

5).The sample collection directly affect the accuracy of testing, so ensure collect sample correctly and fully.

6). Excessive sample adding will not increase sensitivity, but will result in erroneous results. Too few samples may result in false negative results, so please accurately control the amount of sample adding quantity according to the instructions.

7). Avoiding bubbles when adding samples to the sample hole of the test card. When adding samples, please keep the front end of the straw about 1 cm away from the sample hole to ensure accurate adding samples; If the distance is too close, the drop volume may be reduced or the sample size may not be added due to the error in judging the number of drops, which will affect the test effect

8). Used test cards and samples should be considered as pollutants and must be properly disposed of in accordance with relevant local regulations

6. Frequently Asked Questions

1) Limitation of the product

This kit is a qualitative screening reagent, can detect CIV antigen accurately, but if sample is not enough or concentration of antigen is lower than sensitivity, it may appear negative result. A clear clinical diagnosis should be made after taking into account all clinical and laboratory phenomenon. An accurate clinical diagnosis should not be based on a single test result.

2) The sensitivity and specificity of the product.

This rapid test kit has no cross-reaction with similar symptoms to those of Canine distemper virus, adenovirus, parainfluenza virus.

7. Specifications, Storage and Stability.

Package: 10T/box

Store at 2~30 ℃ in a cool, dark and dry place, no frozen. Shelf life: 24 months.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

Website: www.lsybt.com