Green Spring Peste des petits ruminants(PPR) virus antigen rapid test card manufactured by Lvshiyuan

Peste des petits ruminants(PPR) virus antigen rapid test card

Catalog No.: LSY-20089-2

1. Principle

This rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the PPR antigen in serum, plasma, eye and nose secretions. After adding sample, the sample move along with the colloidal gold labeling PPR Monoclonal antibody, if there is PPR antigen in the sample, it combines with antibody on T line and show wine-red color; if there is no PPR antigen, then no color reaction.

2. Components

3. Test procedure

3.1. Wet a cotton swab with normal saline, collect secretions from the eyes, nose, and mouth. Immediately insert the cotton swab into the test tube containing the sample buffer. Rotate the cotton swab on the test tube wall at least 10 times repeatedly and mix the solution. Make the specimen as soluble as possible in the buffer. Squeeze the cotton swab on the wall of the test tube above the liquid surface to make the liquid squeeze out as much as possible. Then discard the cotton swab.

3.2 Or collect serum or plasma, use dropper to drop two drops of sample serum or plasma into the sample buffer and mix it well.

3.3 Open the package, take out test card, put it on clean, flat desk. Use dropper to absorb the sample, adding 5 drops sample into “S” mark well slowly drop by drop.

3.4 At this time, the wine red liquid flowing through the observation window. If the liquid is not flowing, gently press the area between the "sample hole" and the "observation window" to help the liquid release.

3.4 Judge the result in 10-15min, it is invalid beyond 15min.

4. Results judgment

1) Positive: control line and test line are both seen wine red;

2) Negative: only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red. Or only test line is seen wine red.

5. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

6. Notice

1) Do not use test card out of date and bad package. It must be used in 1 hours after take it out;

2) Collected samples should be immediately tested, avoid long-term storage;

3) The collection of samples directly affects the accuracy of detection, so ensure that the samples are collected correctly and adequately.

4) Do not touch the white membrane surface in the middle of test card.

5) Avoid bubble when adding samples to the wells of the test card.

6) Products that have expired cannot be used.

7) Treat the test card and sample at pollutant according to local regulations.

7.Other Questions

1. Which sample works well?

In the early stages of infection, it is better to take blood as a sample. There are no symptoms such as stomatitis, pneumonia, and diarrhea in the early stage of infection. The virus only exists in the blood. If you have suspected that the disease has occurred, it is more convenient to use eye, nose and mouth secretions.

2. What are the limitations of this product?

This reagent is a qualitative screening reagent, which can detect the ruminant petunitis antigen very accurately. However, if the sample is insufficient or the antigen concentration is lower than the sensitivity of the reagent, the negative result may occur. The doctor should make a diagnosis after considering all the clinical symptoms. An accurate clinical diagnosis should not be based on a single test result.

3． What is the sensitivity and specificity of this product?

Compared with other similar rapid test cards, the sensitivity of this test card to detect PPR antigens is> 99%, and the limit of positive results is between 10³-10⁴TCID₅₀ / ml. There are no cross-reactions with similar symptoms of sheep pox, bluetongue, and foot-and-mouth disease.

4． Did you get a positive reaction when tested after the vaccination?

Attenuated vaccines may increase the virus in the body during immunization and may show a positive response. However, this phenomenon disappears 3–10 days after the vaccination.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com