Porcine reproductive and respiratory syndrome (PRRS) virus antigen rapid test kit

Porcine reproductive and respiratory syndrome (PRRS) virus antigen rapid test card

Catalog No. LSY-20012-2

1. Principle

This PRRS Ag rapid test kit is used to detect Porcine reproductive and respiratory syndrome (PRRS) virus antigen in porcine blood, serum, lung tissue and tonsil, and can be used for screening and assisting diagnosis of PRRS virus infection.

This PRRS Ag rapid test kit is made based on the principle of colloidal gold immunochromatography (GICA). After the sample is added to the sampling hole, it moves along the chromatography membrane together with colloidal gold markers. If there is PRRS virus in the sample, it combines with the colloidal gold markers to form a complex. If PRRS virus is not present in the sample, the T-zone does not produce a color response. Line C is the quality control line, line C color shows that the detection card is effective.

2. Specifications: 40 tests/kit

3. Components

1) PRRSV antigen test card 40 pieces

2) Dropper 40 pieces

3) PRRSV Ag sample buffer 40 pieces

4) Cotton swabs 40 pieces

5) Manual 1 piece

4. Sample preparation

4.1 Whole blood

Anticoagulant whole blood collection is used on the same day;

4.2 Serum

Collect blood, put at 37℃ for 1-2h, take the supernatant, centrifuge at 1500r/min for 10min, separate the serum. The serum can store for 2-3 days at 4℃, for long term storage, store at -20℃ in frozen.

4.3. Lung tissue and tonsil: Wipe the surface of lung or tonsil with a cotton swab, wipe at least three times, then put the cotton swab into the sample buffer, discard the tail, tighten the tube cover, fully dissolve, get the sample to be tested.

5. Operation procedures

1) Read the manual carefully and return the test card and sample to room temperature before use.

2) Take out test card, use dropper to absorb the sample, drop 2-3 drops into well mark “S”; or use Micropipette to transfer 70ul sample into well mark “S” .

3) Read the results in 15min at room temperature.

6. Results

Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no PRRSV antigen in sample or the levels is lower than the detection threshold;

Positive(+): Both on T Line and C line being seen red color reaction. It means there is PRRSV antigen in sample;

Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com