LSY-20090-NSP FMD-NSP (3ABC) rapid test kit for ruminants, pig

Foot-and-mouth disease virus nonstructural protein (3ABC)

antibody rapid test kit

Catalog No. LSY-20090-NSP

1. Principle

This FMD-NSP (3ABC) antibody rapid test kit is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the FMD-NSP (3ABC) antibody in whole blood, serum, plasma of pig, cattle and sheep. Applied to the screening of infected animals.

2. Components

3. Test procedure

1) Collect 0.5-1mL whole blood, centrifuge at 3000 r/min for 3~5min to separate serum . Or be static at 4℃ overnight, separate the serum naturally.

2) Take out the test card( return to room temperature firstly if store in cold), put it on desk flatly.

3) Take 30uL serum, add into the sample well mark “S” on test card (Recommended), or use the supplied dropper to add 1 drop of serum into sample well vertically and slowly.

4) Please wait for 10min.

5) Then use the bottle with sample dilution buffer, adding 2 drops of sample dilution buffer into “S” mark well.

6) There will be wine-red liquid flow the observation, wait 10-15min to read the result, the result is invalid after 15min.

4. Results judgment

1) Negative: control line and test line are both seen wine red. It means the antibody level lower than HI titer1:16;

2) Positive: only control line is seen wine red, it means the antibody level higher than or equal to HI titer1:32 ;

3) Invalidation: control line isn’t seen wine red like picture .

5. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

6. Notice

1) Do not use test card out of date and bad package;

2) Do not use pure water, running water and normal saline as negative control;

3) Store at room temperature (< 30℃) is OK. If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.

4) Do not touch the white membrane surface in the middle of test card.

5) To ensure the testing quality, take fresh, unpolluted sample. If the serum can not be tested immediately, store at 4℃ for short time (in 48hours), store at below -20℃ for long-tern storage.

6) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.

7) The antibody level is only related to darkness of T line, no relation with C line. Only the C line is clear to see, it means the test is valid.

8) Treat the test card and sample as pollutant according to local regulations.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com