LSY-20090O FMD serotype O antigen rapid test kit for cattle, sheep, cow, goat and pig

FMD Type O antigen rapid test card for cattle, sheep and porcine

Catalog No. LSY-20090O

1. Principle

This test card is based on colloidal gold immunochromatography technology to detect subtype O of foot-and-mouth disease antigen in samples of feces, saliva or blisters on the skin from cattle, sheep and pig qualitatively. When testing, FMD subtype O Ag in sample combine with antibody coated by colloid gold forming complexes, moving forward to the other head. When reaching the T-line, the specific antibody on the membrane capture the complexes and appear a T line. C line is Control line, C line appears, means the test is valid.

2. Specifications: 40 tests/kit

3. Components

4. Sample preparation

1). Use a cotton swab to take samples of feces, saliva or blisters on the skin;

2). Insert the cotton swab into the sample buffer tube immediately after sampling, rotate the cotton swab repeatedly on the tube wall at least 10 times, and mix the solution to dissolve the sample as much as possible in the sample buffer. Squeeze the cotton swab on the tube wall to allow the liquid to be squeezed out as much as possible and discard the cotton swab.

5. Operation procedures

1) Read the manual carefully and return the test card and sample to room temperature before use.

2) Take out test card, use dropper to absorb the sample, drop 3-4 drops into well mark “S”; or use pipette to take 70uL sample into well mark “S”.

3) Read the results in 15min at room temperature.

6. Results

Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen red color reaction. It means there is no FMD subtype O Antigen in sample or the levels is lower than the detection threshold;

Positive(+): Both on T Line and C line being seen red color reaction. It means there is subtype O of FMD antigen in sample;

Invalid: No color reaction on C Line. It means the operation is wrong or the test card is invalid.

7. Notice

1) The test card can be used only once at room temperature, do not reuse or use test card out of expiry date.

2) Return all reagents and sample into room temperature before use.

3) Be careful when prepare sample, wear glove and mask.

4) The whole blood need add anticoagulants, not frozen; serum sample can store in frozen, avoid repeated freezing and thawing.

5) This product is only for in vitro rapid diagnostic veterinary use, can not be quantified, do only qualitative testing.

6) The product test results for reference only, for confirmation, please refer to the relevant national standard methods.

8. Storage and expiry date

Storage: Store at 4-30 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 24 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

www.lsybt.com