LSY-10068 TheTrenbolon ELISA Test Kit meat safety detection 96 wells/kit ISO certificate

TheTrenbolon ELISA Test Kit

Catalog No. LSY-10068

1. Principle

This test kit is based on the indirect competitive enzyme immunoassay for the detection of theTrenbolon in the sample. The coupling antigen is pre-coated on the micro-well stripes. TheTrenbolon in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Trenbolon antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with theTrenbolon in it. This value is compared to the standard curve and theTrenbolon concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.03ppb

Incubation Temperature: 25℃

Incubation Time: 30min～15min

Detection limit:

Tissue 1.5ppb

Milk,urine 0.6ppb

Milk powder 1.2ppb

Recovery rate

Tissue, milk, urine, milk powder 70%~120%

Cross-reaction rate:

TheTrenbolon 100%

3. Components

4. Materials required but not provided

• Equipments: microplate reader, homogenizer, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), incubator, printer.
• Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl.
• Reagents: Methanol, Sodium hydroxide (NaCl).

5. Sample pre-treatment

Instructions

The following points must be dealt with before the pre-treatment of any kind of sample:

1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2) Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination which interferes with the experimental results.

Solution preparation before sample pre-treatment

1) 50% Methanol solution: 1 part of Methanol + 1 part of deionized water (100ml anhydrous methanol + 100ml deionized water), mix it evenly.

2) 0.01M NaOH solution: Weigh 0.04g solid sodium hydroxide (NaCl) and add deionized water to make the volume to 100ml.

3) Sample diluent: 1 part of 10× Sample diluent + 9 parts of deionized water, mix it evenly.

5.1 Tissue sample preparation

1) Take 1± 0.05 g of the homogenized tissue sample into 10mL/15mL/50mL centrifuge tube;

2) Add 4mL 50% Methanol solution, vortex for 3min; centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;

3) Take 100 µl up-layer clear liquid, add 900 µl Sample diluent,mix it evenly;

4) Take 50uL above liquid for analysis.

Fold of dilution of the sample: 40

5.2 Milk and Urine sample preparation

1) Take 1ml of the milk or urine sample into 10mL/15mL/50mL plastic centrifuge tube;

2) Add 4mL 0.01M NaOH solution, vortex for 3min;

3) Centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;

4) Take 200 µl up-layer clear liquid, add 600 µl Sample diluent,mix it evenly;

5) Take 50uL above liquid for analysis.

Fold of dilution of the sample: 20

5.3 Milk powder sample preparation

1) Take 1g of the milk powder sample into 10mL/15mL/50mL plastic centrifuge tube;

2) Add 4mL 0.01M NaOH solution, vortex for 3min;

3) Centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;

4) Take 100 µl up-layer clear liquid, add 900 µl Sample diluent,mix it evenly;

4) Take 50uL above liquid for analysis.

Fold of dilution of the sample: 40