LSY-10063 Nicarbazine ELISA Test Kit for feed

Nicarbazine ELISA Test Kit

Catalog No. LSY-10063

1. Principle

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Nicarbazine. The coupling antigen is pre-coated on the micro-well stripes. The Nicarbazine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Nicarbazine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Nicarbazine in the sample. This value is compared to the standard curve and the Nicarbazine residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubator temperature: 25℃

Incubator time: 30min～15min

Detection limit:

Tissue……………………………...….10ppb

Feed……………………………...…....20ppb

Cross-reaction rate:

Nicarbazine ..........................................100%

Recovery rate:

Tissue, Feed………………………70%-120%

• Components

4. Materials required but not provided

1) Equipment: ELISA Reader (450 nm/630nm), homogenizer, shaker, centrifuge, balance: 0.01g quantity sensitive, incubator, graduated pipettes, printer

2) Micropipettes: single-channel 20ml ~ 200ml, 100ml ~ 1000ml, multi-channel 30～300 μl

3) Reagents: Acetonitrile.

• Sample pre-treatment

Instructions (The following points must be dealt with before the pre-treatment)

• Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2) Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

Samples Preparation

• Preparation of Tissue sample

• Take 1.0±0.05g tissue sample into 10ml centrifuge tube, add 2ml acetonitrile, vortex for 5min;
• Centrifuge at above 4000r/min at room temperature(20℃-25℃) for 5 min;
• Take 50ul supernatant, add 950ul Sample dilution, shake violently to mix for 30s;
• Take 50μl to test.

Dilution factor: 40

• Preparation of Feed sample

• Take 5.0±0.05g feed sample into 50ml centrifuge tube; add 20ml acetonitrile, then vortex for 5min;
• Centrifuge at above 4000r/min at room temperature(20℃-25℃) for 5min;
• Take 50ul supernatant, add 950ul Sample dilution, shake violently to mix for 30s;
• Take 50μl to test.

Dilution factor: 80