LSY-10057 Azithromycin ELISA Test Kit for tissue, egg, milk ISO certificated

Azithromycin ELISA Test Kit

Catalog No. LSY-10057

1. Principle

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Azithromycin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Azithromycin in the sample and the coupling antigen pre-coated on the micro-well stripes compete for the anti-Azithromycin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Azithromycin in it. This value is compared to the standard curve and the Azithromycin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.05ppb

Incubation Temperature: 25℃

Incubation Time: 30min～15min

Detection limit:

Tissue, egg, milk 0.5ppb

Recovery rate

Tissue, egg, milk 70%~120%

Cross-reaction rate:

Azithromycin 100%

Erythromycin < 1%

Tylosin 30%

Tilmicosin 23%

3. Components

4. Materials required but not provided

• Equipments: microplate reader, homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, and balance( a sensibility reciprocal of 0.01 g), incubator.
• Micropipettors: single-channel 20-200 µL, 100-1000 µL, and multi-channel 30～300 μl.
• Reagents: Methanol

5. Sample pre-treatment

Instructions

The following points must be dealt with before the pre-treatment of any kind of sample:

1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2) Before the experiment, each experimental equipment must be checked to be clean and should be re-cleaned if necessary, in order to avoid the contamination which interferes with the experimental results.

Solution preparation before sample pre-treatment

10% Methanol: 1 part of Methanol + 9 parts of deionized water.

5.1 Tissue(chicken, duck), egg, milk sample preparation

1) Take 1± 0.05 g of the homogenized fresh sample into 50mL centrifuge tube, add 5mL 10% Methanol, vortex for 3min; centrifuge at above 4000 r/min at room temperature (20 - 25 ℃) for 5 minutes;

2) Take 50uL up-layer liquid for analysis.

Fold of dilution of the sample: 5