LSY-20005-2 Malachite green rapid test strip (Tissue)

Malachite green rapid test strip (Tissue)

Catalog No. LSY-20005-2

• Specifications

10 strips/tube

2. Brief

This product is used for testing Malachite green and Leucomalachite Green in Tissue (include Pork, Chicken, Fish, Shrimp etc.) sample qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Tissue: 1ng/g (1ppb)

4. Contents

5. Principle

The Malachite green rapid test card is based on competitive inhibition immuno-chromatographic principle. In the flow process, Malachite green in the sample combined with Malachite green specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Malachite green-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Malachite green residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Malachite green residue in sample, C line will appear, it means the test is valid.

6. Materials required but not provided

1) Equipments: Homogenizer, nitrogen-drying device, vortex(optional), centrifuge, Micropipette, balance (a reciprocal sensibility of 0.01 g);

2) Consumables: 15ml centrifuge tube, disposable tips.

7. Sample preparation

1) Take 3±0.05g homogenized tissue sample into 15ml centrifuge tube, add 4ml Reagent 1, then add 4ml reagent 2, shake by hand or by vortex strongly for 2min (shake by hand up-and-down strongly), add 1 bottle of reagent 3, shake strongly for 2min (shake by hand up-and-down strongly), then centrifuge at 4000r/min for 5min;

2) Transfer 1.5ml up-layer clear liquid into another centrifuge tube, add 100ul reagent 4, shake for 30S. Blow to dry at 56℃ by Nitrogen or air(or use hair dryer);

3) Add 0.3ml reagent 5, shake for 30S (or repeatedly blow and beat to redissolve) to fully dissolve the residue on wall of centrifuge tube, absorb 120ul down-layer liquid as sample, ready to test.

8. Operation procedures

8.1 Read the instruction carefully before use. Return test strips and sample into room temperature.

8.2 Take out tubes, open and take out required test strips and microwells, making proper marks. Please use them within 1h. Seal the cup of unused microwells and tubes immediately, avoid moisture.

8.3 Take 120ul of the test samples into the microwells, then repeatedly absorb for many times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).

8.4 Incubate for 5min at room temperature (20-25℃), insert the test strips into the microwells with the "MAX" end fully dipped in to the mixture solution.

8.5 Incubate for 5min at room temperature again, read the result, it is invalid in other time.

9. Test Result Interpretation

9.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Malachite green residue in sample or the residue is lower than detection limit.

9.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Malachite green residue is equal to or higher than detection limit.

9.3 Invalidation: C line isn’t seen wine red. It means the test strip is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

10. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

3) Test again for positive result;

4) Please contact the supplier for any questions.

11.Specificity

Test 500ppb Tetracyclines, Quinolones and Sulfonamides etc. The result if Negative.

12. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788

Fax 86-755-28938800

Email: info@lsybt.com

Website: www.lsybt.com