LSY-20006 Aflatoxin B1(AFB1) rapid test strip

Aflatoxin B1 (AFB1) rapid test strip

Catalog No. LSY-20006

1. Brief

This product, based on competitive inhibition of colloidal gold immunochromatography principle, is used for testing Aflatoxin B1 (AFB1) residue in grain, feed and edible oil sample etc.

• Technical indicator

2.1 Sensitivity: 2ppb (ng/ml)

Detection limit= Sensitivity* Sample dilution factor

2.2 Detection limit:

Grain, feed, edible oil: 10ppb

Grain, feed(Need blow to dry): 5ppb

3. Principle

The Aflatoxin B1 rapid test strip is an immuno-chromatographic test, using colloidal gold immunoassay method. It relies on the competition between Aflatoxin B1 residues in the sample and the Aflatoxin B1 on T line, to the limited amount of dye-antibody. As a sufficient amount of drug in the sample is presenting, the drug will saturate the antibody. Consequently, it will show an extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a negative sample (or the amount of the drug is lower than the minimum detectable concentration), it will generate two obvious lines in both the T and C line section.

4. Contents

1) AFB1 Test card 50 pieces

2) Manual 1 piece

5. Material required but not provided

5.1 Instrument: homogenizer, oscillator, centrifuge, scale pipette, balance (0.01g)

5.2 Micro-pipettes: Single channel 20ul-200ul, 100ul-1000ul

5.3 Reagent: methanol, n-hexane

6. Sample preparation

6.1 Note: The experimental utensil must be clean and use disposable tips to avoid pollution and affect the result.

6.2 Solution preparation

Sample extracting buffer: 70% methanol: V_methanol:V_{deionized water}=7:3

6.3 Sample treatment procedure

6.3.1 Grain, feed

1) Weigh 2g crushed sample into centrifuge tube, add different volume of Sample extracting buffer upon detection limit request accoring to following Tablet

Shake for 5min, Centrifuge at 4000r/min at room temperature for 5min;

2) Take 0.1ml up-layer clear liquid, add 0.4ml deionized water, mix it evenly, ready for use.

6.3.2 Grain, feed(need blow to dry)

If require detection limit of 5ppb, operate as following:

1) Weigh 2g crushed sample into centrifuge tube, add 4ml Sample extracting buffer, Shake for 5min, Centrifuge at 4000r/min at room temperature for 5min;

2) Take 1ml up-layer liquid, blow to dry at 50-60℃ by Nitrogen or air;

3) Firstly add 0.25ml Sample extracting buffer to dissolve the dry residue, shake strongly for 2min; then add 1ml deionized water, mix it evenly, ready to use. (Note: For this step, firsly add Sample extracting buffer, then add deionized water, it can’t change turn)

Sample Dilution Factor: 2.5 Detection limit: 5ppb

6.3.3 Edible oil (vegetable oil, sesame oil, salad oil, peanut oil, etc.)

1) Weigh 2g sample into centrifuge tube, add different volume of Sample extracting buffer upon detection limit request accoring to following Tablet

Then add 8ml n-hexane, shake for 5min, Centrifuge at 4000r/min at room temperature for 5min;

2) Remove up-layer liquid, take 0.1ml down-layer liquid, add 0.4ml deionized water, mix it evenly, ready to use.

7. Operation procedures

7.1 Take out the test card from Aluminum foil bag, put it on flat, clean desk.

7.2 Use dropper to drop 2~3 drops of sample drop-by-drop and slowly(about 60 ul, avoid bubble) in the Sample collect region vertically.

7.3 Wait for 8~10 min at room temperature to read the result. (Result beyond10 minutes can be as reference only)

8. Test Result Interpretation

8.1 Negative: Red T line appears. It means there is no aflatoxin B1 residue in sample or the residue is lower than detection limit.

8.2 Positive: Red T line is invisible. It means the aflatoxin B1 residue is higher than detection limit.

8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the local distributor.

9. Precautions

1) Do not use test card out of expiry date and in broken package;

2) Return the test card to room temperature if store in cold before use, use the test card soon to avoid moisture after open the package;.

3) Do not touch the white membrane surface in the middle of test card;

4) Do not mix use the dropper to avoid cross pollution.

5) The sample liquid need to be clear, no turbid particles, no bacterial contamination, otherwise easily lead to blocking, color‘s not obvious etc., thus affecting the results

10. Storage and expiry date

Storage: Store at 2-30 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.