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Sulfonamides residues (SAs) rapid test strip (Tissue)
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Product Details:
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Detailed Product Description
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Sulfonamides residues (SAs)rapid teststrip (Tissue)
Catalog No. LSY-20040
1. Brief
This product is used for testing Sulfonamides residue in tissue sample. The total test time only need 30~60 min.
2. Detection limit in milk
Sulfadiazine (SD or SDZ) | 10 ppb |
Sulfamerazine (SM1) | 10 ppb |
Sulfamonomethoxine (SMM) | 10 ppb |
Sulfathiazole (ST) | 10 ppb |
Sulfadimethoxine(SDM) | 10 ppb |
Sulfamethoxypyridazine (SMP) | 15 ppb |
Sulfaquinoxaline(SQX) | 10 ppb |
Sulfamethoxazole (SMZ) | 30 ppb |
Sulfanethazine(SM2) | 15 ppb |
Sulfamethoxydiazine(SMD) | 10 ppb |
3. Specifications: 50 tests/kit
4. Principle
The Sulfonamides rapid test strip is an immuno-chromatographic test, using colloidal gold immunoassay method. It relies on the competition between Sulfonamides residues in the sample and the Sulfonamides on T line, to the limited amount of dye-antibody. As a sufficient amount of drug in the sample is presenting, the drug will saturate the antibody. Consequently, it will show an extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a negative sample (or the amount of the drug is lower than the minimum detectable concentration), it will generate two obvious lines in both the T and C line section.
5. Contents
1 | Sulfonamides test card | 50 pieces |
2 | Manual | 1 piece |
3 | Dropper | 50 pieces |
4 | Desiccant | 1 piece/bag |
5 | Disposable gloves | 3 pieces |
6 | Redissolving solution | 1 bottle |
6. Materials needed but not provided.
Equipment:homogenizer, nitrogen-drying device, vortex, centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g).
Reagents:5ml centrifuge tube, tips, ethyl acetate, n-Hexane.
7. Sample preparation
Tissue
Take 3±0.05g homogenized tissue sample, add 3ml deionized water, then add 4ml ethyl acetate, shake for 3min, centrifuge at 4000r/min for 5min, take 2ml supernatant into another 5ml centrifuge tube, blow to dry at 56℃ by Nitrogen or air (or use hair dryer). Add 0.4ml redissolving solution to redissolve, then add 1ml n-Hexane, shake and mix for 1min, centrifuge at 4000r/min for 5min, discard up-layer liquid, take down-layer liquid to test.
8.Operation procedures
8.1 Return test card and sample into room temperature.
8.2 Take necessary strips on desk, use it in 1 hour.
8.3 Put the test card flatly, use dropper to drop2dropsof liquid in the Sample collect region vertically.
8.4 Wait for 5~8 min to read the result. It is invalid in other time.
9.Test Result Interpretation
9.1 Negative: Red T line appears. It means there is no Sulfonamides residue in sample or the residue is lower than detection limit.
9.2 Positive: Red T line is invisible. It means the Sulfonamides residue is higher than detection limit.
9.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
10.Precautions
1) The test card can be used only once at room temperature, do not use test card out of expiry date.
2) Every test card and dropper is single use only, to avoid cross pollution.
3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.
4) Tap water, distilled water and deionized water can not be as negative control.
5) Please contact the supplier for any questions.
11.Storage and expiry date
Storage:Store at 4-30 ℃ in dark, sealed, dry place,no frozen.
Expiry date:12 months; date of production is on box.
Contact Details