Tetracyclines rapid test strip for honey detection

Tetracyclines rapid test strip

Catalogue No.: LSY-20046-1

1. Brief

This product is used for testing Tetracyclines residue in honey samples (raw honey and concentrated honey). The whole test only need about 25 min, The sensitivity of Tetracyclines is5μg/kg（ppb）, suitable for all kinds of enterprises and Inspection agency.]

2. Components

1) Tetracyclines colloidal gold rapid test strip

(Including tablet, gold label well, dropper, desiccant) 50 pieces/kit

2) TCs PBST Buffer 1 bottle/kit

3) Manuals 1 piece/kit

3. Materials needed but not provided

15ml graduated centrifuge tube, balance (a sensibility reciprocal of 0.01 g), Micropipettes

4. Sample preparation

For non-crystallized honey sample, stir directly to even; For crystalline sample, incubate at 60℃~ 80℃ hot water in a close environment, stir the honey until all the samples melted.

1) Weigh 5g honey sample into 15ml graduated centrifuge tube;

2) Add 5ml Ethyl acetate, shake up-and-down to even, centrifuge at 4000 rpm at room temperature for 5 min, take 4ml supernatant into 5ml graduated centrifuge tube, blow to dry by nitrogen or air at 60℃;

3) Add 0.3ml TCs PBST Buffer to the dry tube, use dropper drop the liquid and absorb liquid in the tube to wash the inner well.

4) Use Micropipettes to absorb 150ul solution into gold label well, ready to test.

5.Operation procedures

1) Read the Manual, return the test strips and sample solution to room temperature. （20℃~30℃）before testing.

2) Take out the test strip, put it on desk flatly, as the following picture on right (Use it immediately after opening the package.)

3) Absorb 150μL up-layer sample solution into gold label well, wait for 2 min, use small dropper to blow and beat until the red material is completely dissolved, wait to react for 2min,

4) Use dropper absorb all sample from the well, vertically add it into sample collecting well (as following picture), timing after adding sample.

5) Read the result in 5-8 minutes, otherwise it is invalid.

Note: S means Sample Colleting Region

C means Control Line

T means Test Line

6.Test Result Interpretation

Negative Positive Invalid

6.1 Negative (—): T line appears (T line, near Sample Collecting Region), T line is darker or has the same color with C line, it means the TCs concentration is lower than 5μg/kg or no TCs residue.

6.2 Positive (+): T line is lighter than C line or T line is invisible, it means the TCs concentration is higher than 5μg/kg. The more lighter of T line compared with C line, the higher content of TCs in sample.

6.3 Invalid: C line is invisible, maybe it is the operation error or test strip is invalid. Read the Instruction, use new test strip to test again.

7.Precautions

1) The test card is disposable.

2) Do not use test card out of expiry date, proper handling the waste.

3) Do not touch the white membrane surface in the middle of test card.

4) Do not repeat use the dropper, to avoid cross-reaction.

5) Do not eat any of the reagents.

6) Contact the supplier for any questions.

7) If need to test standard directly, please use our PBST buffer to prepare.

8. Specificity

This product has no cross-reaction with Streptomycin, Tetracyclines, Sulfonamides etc

9. Precision

Use this product and Tetracyclines ELISA test kit at the same time to test 566 samples including 188 negative samples and 378 positive samples, the results show that the coincidence rate is 98.5% between this product and Tetracyclines ELISA test kit.

10.Storage and expiry date

Storage:Store at 4-30 ℃in dark,no frozen.

Expiry date:12 months; date of production is on box.