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Product Details:
Payment & Shipping Terms:
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Specifications: | 96 Wells/kit | Sensitivity: | 0.05ppb |
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Recovery Rate: | 90±30% | Shelf Life: | 12 Months When Properly Stored |
Sample Performance: | Honey | MOQ: | 1 Kit |
High Light: | Metronidazole ELISA kit,Honey test kit,honey elisa kit |
Metronidazole ELISA Test Kit (Honey)
Catalog No. LSY-10048
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Metronidazole in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Metronidazole in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Metronidazole antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Metronidazole in it. This value is compared to the standard curve and the Metronidazole concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.05ppb
Detection limit
Honey: about 0.1ppb
Note: ppb= ng/ml or ng/g
Cross-reaction rate
Metronidazole 100%
Ornidazole 83%
Secnidazole 110%
Metronidazole-OH <1%
Tinidazole <1%
Dimetridazole <1%
Recovery rate: 90±30%
3. Components
4. Materials required but not provided
5. Sample pre-treatment
Instructions
The following points must be dealt with before the pre-treatment of any kind of sample:
Solution preparation before sample pre-treatment:
5.1 Sample preparation
Honey
1) Weigh 2.0± 0.05g honey sample into 50ml plastic centrifuge tube;
2) Add 2ml 0.2M Phosphate buffer, then add 8ml Ethyl acetate, shake strongly for 5min (or use vortex for 3min) to make sample contact with organic phase completely;
3) Centrifuge at above 3000 g at room temperature for 5min;
4) Transfer 4ml up-layer clear liquid into 5ml centrifuge tube (note: do not take down-layer water phase), blow to dry in 50-60℃ water bath;
5) Add 1ml Hexane and 0.5ml Redissolving solution accordingly, shake strongly for 2min (or use vortex for 1min);
6) Centrifuge at above 3000 g at room temperature for 5min;
7) Discard up-layer organic phase, take 50ul down-layer liquid to test.
Fold of dilution of the sample: 0.5
6. ELISA procedures
6.1 Instructions
1) Bring all reagents and micro-well strips to the room temperature (20-25 ℃) before use;
2) Return all reagents to 2-8 ℃ immediately after use;
3) The reproducibility of the ELISA analysis, to a large degree, depends on the consistency of plate washing. The correct operation of plate washing is the key point in ELISA the procedures;
4) For the incubation at constant temperatures, all the samples and reagents must avoid light exposure, and each microplate should be sealed by the cover membrane.
6.2 Operation procedures
7. Result judgment
There are two methods to judge the results: the first one is the rough judgment, while the second is the quantitative determination. Note that the OD value of the sample has a negative correlation with the Metronidazole concentration.
7.1 Qualitative determination
The concentration range (ng/mL) of Metronidazole can be obtained from comparing the average OD value of the sample with that of the standard solution. Assuming that the OD value of the sampleⅠ is 0.3, and that of the sampleⅡ is 1.0, the OD value of standard solutions is: 2.243 for 0ppb, 1.816 for 0.05ppb, 1.415 for 0.15ppb, 0.74 for 0.45ppb, 0.313 for 1.35ppb, 0.155 for 4.05ppb, accordingly the concentration range of the sampleⅠ is 1.35 to 4.05ppb, and that of the sampleⅡ is 0.05 to 0.45ppb.
7.2 Quantitative determination
The mean values of the absorbance values is obtained for the average OD value (B) of the sample and the standard solution divided by the OD value (B0) of the first standard solution (0 standard) and subsequently multiplied by 100%, that is,
Percentage of absorbance value = | B | ×100% |
B0 |
B—the average OD value of the sample or the standard solution
B0—the average OD value of the 0 ng/mL standard solution
Draw the standard curve with the absorption percentages of the standard solution and the semilogarithm values of the Metronidazole standard solution (ng/mL) as Y- and X-axis, respectively. Read the corresponding concentration of the sample from the standard curve by incorporating its absorption percentage into the standard curve. The resulting value is subsequently multiplied by the corresponding dilution fold, finally obtaining the Metronidazole concentration in the sample.
Using the professional analyzing software of this kit will be more convenient for the accurate and rapid analysis of a large amount of samples. (Please contact us for this software).
8. Precautions
9. Storage and expiry date
Storage: store at 2-8 ℃, not frozen.
Expiry date: 12 months; date of production is on box.