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Product Details:
Payment & Shipping Terms:
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Specifications: | 96 Tests/kit | Sample Performance: | Honey, Tissue, Egg, Milk |
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Shelf Life: | 12 Months When Properly Stored | Incubation Time: | 5min-5min |
Brand: | Green Spring |
Quinolone rapid test dipstick (Egg)
Tissue, milk, honey sample is available
Catalog No. LSY-20047
1. Brief
This product is used for testing Quinolone residue in egg, duck egg sample qualitatively, the whole test procedure only need 10min, easy to operate with high sensitivity.
2. Detection limit in milk (ng/ml or ppb)
Enrofloxacin | 20ppb | Danofloxacin | 40ppb |
theCiprofloxacin | 20ppb | Flumequine | 20ppb |
Norfloxacin | 20ppb | By difloxacin | 40ppb |
Pefloxaein | 20ppb | Lomefloxacin | 40ppb |
Sarafloxacin | 30ppb | Marbofloxacin | 40ppb |
Ofloxacin | 35ppb | Nadifloxacin | 40ppb |
3. Specifications: 8 dipsticks/bottle, 12 bottles/box
4. Principle
The Quinolone rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Quinolone in the sample combined with Quinolone specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Quinolone -BSA conjugate on Test line of NC membrane, lead to the color change of Test line.
When the sample has no Quinolone residue or concentration lower than detection limit, T line is darker than C line or
has same color with C line; when the concentration is equal to or higher than detection limit, T line is lighter than C line or T line has no color. No matter whether there is Quinolone residue in sample, C line will appear, it means the test is valid.
5. Contents
1 | Quinolone test dipsticks | 8 dipsticks/bottle, 12 bottles |
2 | Red powder micro-wells | 96 wells |
3 | Manual | 1 piece |
4 | Desiccant | 2 pieces/bottle |
Sample preparation:
Take 1g fres even egg sample, add 3ml pure water, shake with hand or by oscillator strongly for 3 mins, ready to use.
6. Operation procedures
6.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
6.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
6.3 Take 200ul of the test samples into the micro-wells, then repeatedly absorb for 5 times, mix the sample with the reagent in themicro-wells completely until no solid judge by eyes (this is a very important step).
6.4 Incubate for 5min at room temperature (20-25℃), then insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
6.5 Incubate for 5-8min at room temperature again, read the result, it is invalid in other time.
7. Test Result Interpretation
7.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Quinolone residue in sample or the residue is lower than detection limit.
7.2 Positive: C line appears,T line is obviously lighter than C line or T line is invisible. It means the Quinolone residue is equal to or higher than detection limit.
7.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
Negative Negative Positive Postive Invalid Invalid
8. Specificity
This product has no cross reaction with Tetracyclines, Sulfonamides, Streptomycin etc.
9. Precautions
1) The test card can be used only once at room temperature, do not use test card out of expiry date.
2) Do not repeat use the disposable tips to avoid cross pollution.
3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.
4) Tap water, distilled water or deionized water can not treat as negative control.
5) Please contact the supplier for any questions.
10. Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.