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Highlight: | Simian B Virus test kit,elisa assay kit,animal disease diagnostic testing |
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Brand: Green Spring |
Simian B Virus antibody ELISA kit Catalogue No.:HF-1001 |
Brief
B virus (Cercopithecine herpesvirus 1) is a member of subfamily Alphaherpesvirinae and is classified into the genus Simplexvirus together with human herpes simplex virus 1 (HSV-1) and HSV-2. It is enzootic in rhesus monkeys (Macaca mulatta), cynomolgus monkeys (Macaca fascicularis), and other Asiatic species of the genus Macaca. Clinical manifestation of B virus infection is generally mild in these natural hosts. Human infections with this virus, although the incidence is quite low, result in severe and sometimes fatal encephalitis. Since nonhuman primates are important experimental animals for biomedical research, it is very important to reduce the risk of B virus infection in animal handlers, laboratory workers, and researchers. For this purpose, efforts have been made to establish B virus-free monkey colonies. Serological diagnosis of B virus infection in macaques is usually conducted by antibody detection using enzyme-linked immunosorbent assay (ELISA).
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Principle
Specific genetic engineering antigen is coated on microtiter plate. After bounding with antibodies in the sample, enzyme conjugate is added, reacts with the antibody-antigen complex and generates a new substrate, unreacted materials are washed away. The addition of TMB solution leads to a color change, and anti-SBV antibodies in the serum are detected.
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Specifications
Cutoff value=0.14+N ( N is average A value of negative control ,must be less than 0.10,if less than 0.02, incorporate 0.02 to calculate cutoff value)
If sample A value is less than cutoff value, judged as negative, higher or equal cutoff value, positive.
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